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Stem-like cells cultured from human brain glioma tissues show high invasiveness in vitro
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Abstract:
Objective:To isolate and culture human brain glioma stem-like cells (GSLCs), and to determine the invasiveness of GSLC in vitro. Methods: Glioma cell spheres were serum free cultured from 8 surgical specimens of glioma inpatients from October 2008 to January 2009 in the Department of Neurosurgery, First Hospital of China Medical University. CD133 expression was identified by immunocytochemistry assay, and the expressions of GFAP and TU-20 in differentiated glioma cell spheres were detected under an immunofluorescence microscope. With matrigel invasion assay, invasiveness of glioma sphere cells was determined and compared with that of primary glioma cells. Results: Glioma cell spheres were cultured successfully. These cells were proved to express stem cell marker CD133, capable of renewal and proliferation, and can differentiate to glia and neurons with positive expressions of GFAP and TU-20, indicating the characteristics of GSLC. The invasiveness of glioma spheres cells in vitro was higher than that of primary glioma cells (261.23±87.20 vs 116.08±63.88, P<0.01). Moreover, glioma spheres cells preferred to aggregate and reform new spheres after travelling through the matrigel. Conclusion:〗GSLCs are successfully cultured from human glioma tissues, which show higher invasiveness in vitro. GSLC may participate in glioma invasion and metastasis.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81000565)
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