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Reovirus induces apoptosis of breast cancer mammosphere cells
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Abstract:
Objective:To investigate the effect of reovirus on apoptosis of breast cancer mammosphere cells. Methods:MCF-7 and BT474 cells were cultured in stem cell culture medium to generate mammospheres. Then the mammospheres were infected by reovirus or treated with epirubicin, The proportion CD44+CD24-/low cells in the mammosphere cells from MCF-7 and BT474 cells was detected by flow cytometry (FCM); the apoptosis of mammosphere cells was detected by TUNEL; and the expression of Ras in MCF-7 cells and the mammosphere cells was examined by Western blotting. Results: The infection rates of reovirus on MCF-7 and BT474 mammosphere cells were significantly higher than those on MCF-7 and BT474 cells (all P<0.05). The proportions of CD44+CD24-/low cells in the mammosphere cells from MCF-7 and BT474 cells after treatment with epirubicin were improved greatly (\[8.1±0.4\]% vs \[18.0±4.5\]%, P<0.01; \[0.6±0.2\]% vs \[0.9±0.13\]%, P<0.05), while the proportions of CD44+CD24-/low cells in the mammosphere cells after treatment with reovirus were decreased apparently (\[8.1±0.4\]% vs \[0.8±0.2\]%, P<0.05; \[0.6±0.2\]% vs \[0.2±0.1\]%, P<0.05). The apoptosis rates of the mammospheres cells from MCF-7 and BT474 cells rose up from (1.9±0.21)% to (5±1.4)% (P<0.05) and from (0.2±0.1)% to (1.0±0.16)% (P<0.05) after reovirus treatment. Ras levels in MCF-7 cells and the mammosphere cells were almost same high. Conclusion: Compared with that of epirubicin, reovirus has a higher inhibitory effect on breast cancer mammosphere cells, while reovirus distinctively induces apoptosis of mammosphere cells.
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