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Effects of resveratrol on proliferation, adhesion and invasion of A549 lung adenocarcinoma cells
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Abstract:
Objective: To study the effect of resveratrol (Res) on proliferation, adhesion and invasion of A549 lung adenocarcinoma cells. Methods: A549 cells were treated with different doses of Res. The cell proliferation, cell cycle and apoptosis, adhesive ability, and invasion of A549 cells were examined by methyl thiazolyltetrazolium (MTT) assay, flow cytometry, Boyden chamber assay and Transwell assay respectively (3T3 cells as a normal cell control). The expressions of MMP-2 and TIMP-2 proteins in A549 cells were assayed by immunofluorescent cytochemistry.Results: Res inhibited the proliferation of A549 cells in a dose (20-80 μmol/L) and time (0-72 h) dependent manner. No significant inhibitory effect on 3T3 cell proliferation was observed at the same dose. Res induced the apoptosis of A549 cells with the apoptotic rates of (34.9±0.91)%, (41.33±0.13)%, (45.47±0.87)% and (59.46±0.59)% respectively when Res being 10, 20, 40, 80 μmol/L. A549 cells were arrested at the S phase of cell cycle, and the cell proportion in the S phase was (56.41±167)% when treated with 20 μmol/L Res, the adhesion and invasion ability of A549 cells was effectively decreased after treatment with 20 μmol/L -80 μmol/L Res (P<0.05), accompanied by a significant down-regulation of MMP-2 expressions and up-regulation of TIMP-2 expressions. Conclusion:Res can inhibit the proliferation, adhesion and invasion of A549 lung adenocarcinoma cells, which may be related with the bilateral regulation on MMP-2 and TIMP-2 expressions.
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Project Supported:
Project supported by the Foundation for Medical Guide Program of Shanghai Science and Technology Commission (No. 09411964800)
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