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Leptin promotes migration and invasion of human glioma U87MG cells
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Abstract:
Objective: To investigate the effect of leptin on the migration and invasion of human glioma U87MG cells, and explore its molecule mechanism. Methods: U87MG cells were treated with leptin, the cell migration was analyzed by cell scratch assay, cell invasion was detected by Transwell, and mRNA and protein expressions of MMP-2 and MMP-9 in U87MG cells were observed by RT-PCR and Western blotting assay. Results: Leptin significantly promoted migration (\[152.42±3.29\] vs \[83.24±2.61\] μm, P<0.05), and invasion (\[31.78±5.04\] vs \[17.03±2.41\]/field, P<0.05) of U87MG cells; the mRNA and protein expressions of MMP-2 and MMP-9 were significantly upregulated by leptin in U87MG cells (mRNA: \[0.76±0.04\] vs \[0.35±0.02\], \[0.84±0.02\] vs \[0.41±0.06\], P<0.05; protein: \[079±0.03\] vs \[0.23±0.01\], \[0.81±0.05\] vs \[0.39±0.03\], P<0.05); MMP inhibitor GM6001 reversed U87MG cell migration (\[82.05±2.98\] vs \[81.76±3.25\] μm, P>0.05\] and invasion (\[19.23±2.46\] vs \[18.02±1.98\]/field, P<0.05) induced by leptin. Conclusion: Leptin can promote the invasion and migration of human glioma U87MG cells, which might related with the upregulation of MMP-2 and MMP-9.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81101906), and the Foundation of Shanghai Science and Technology Committee (No. 8411953600)
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