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MicroRNA-7 sensitizes the chemosensitivity of non-small cell lung cancer cells to epirubicin treatment
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Abstract:
Objective:To investigate the effect of microRNA-7 (miR-7) on the chemosensitivity of non-small cell lung cancer A549 and H1299 cells to epirubicin (EPI) treatment and its molecular mechanism. Methods: After being treated with EPI and transfected with miR-7 mimics alone or in combination, the cell proliferation of A549 and H1299 cells was measured by CCK-8; the cell apoptosis was detected by Annexin V/PI staining; and the expressions of EGFR and Raf-1 mRNA were examined by real-time PCR. Results: Both EPI treatment and miR-7 mimics transfection inhibited the proliferation of A549 and H1299 cells (P<0.05), while EPI (50-400 ng/ml) combined with miR-7 mimics had a stronger inhibitory effect on the proliferation of A549 and H1299 cells than EPI treatment alone (P<0.05). The percentages of apoptotic cells was significantly increased in the combination treatment group by (54.9±0.4)% in A549 cells and (672±0.5)% in H1299 cells (P<0.01) compared with that of the EPI treatment group, and the expressions of EGFR and Raf-1 mRNA were also downregulated by (68.0±6.0)% and (78.2±3.9)% in A549 cells, (94.8±6.2)% and (87.8±4.3)% in H1299 cells after the combination treatment of miR-7 mimics and EPI compared with that of the EPI treatment alone group (P<0.01).Conclusion: miR-7 enhances the inhibitory and pro-apoptosis effect of EPI on A549 and H1299 cells through the downregulation of EGFR and Raf-1 mRNA.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81072408), and the Foundation of Shanghai Science and Technology Committee (No. 10JC1401100)
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