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Modification by EGFR small peptide ligand enhances transfection efficiency of cationic liposome on tumor cells
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Abstract:
Objective:To modify PEGylated cationic liposome was modified by small peptide ligand D4 of epidermal growth factor receptor (EGFR), and study its effect on enhancing the transfection efficiency of plasmid DNA and siRNA in tumor cells. Methods: D4 was conjugated to the end of DSPE-PEG2000 to modify the PEGylated cationic liposome, and the effects of this vector system on transfection efficiency of plasmid DNA in EGFR highly-expressed human non-small lung cancer H1299 cells was examined. Luciferase expression in plasmid DNA tranfected-H1299 cells was observed by Siriu sillumination apparatus, and the fluorescence intensity of FAM-siRNA tranfected-H1299 cells was detected by fluorescence microscopy. Results: In prepared liposome/plasmid DNA, with increasing of electric charge ratios, the particle diameter of complex was gradually decreasing and Zeta electric potential was increasing. Compared with non-targeted liposome, liposome modified by D4 significantly increased the expression of luciferase in H1299 cells after plasmid DNA tranfection (P<0.05, or P<0.01); the transfection efficiency of D4-modified liposome with different electric charge ratios on H1299 cells was significantly increased compared with un-modified liposome (P<0.05, or P<0.01). In addtion, in the transfection of FAM-siRNA, an enhanced fluorescence intensity of FAM was observed in the D4-modified liposome group under a fluorescence microscope. Conclusion: D4-modified cationic liposome can improve the transfection efficiency of plasmid DNA and siRNA in EGFR highly-expressed tumor cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30801446)
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