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BRF2 gene expression in esophageal squamous cell carcinoma tissues and its clinical significance
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Abstract:
Objective:To investigate the expression of TFIIB-related factor 2 (BRF2) in human esophageal squamous cell carcinoma tissues, adjacent esophageal cell carcinoma tissues and normal esophageal tissues, so as to analyze the role of BRF2 in the development and progression of esophageal squamous cell carcinoma. Methods: Samples were obtained from 74 esophageal squamous cell carcinoma patients who had been surgically treated during Jan. 2007 to Jan. 2008 in Qilu Hospital of Shandong University. Immunohistochemisty and RT-PCR were applied to detect BRF2 expression in esophageal squamous cell carcinoma tissues, adjacent esophageal squamous cell carcinoma tissues and normal esophageal tissues. Results: The expression of BRF2 mRNA in esophageal squamous cell carcinoma and adjacent esophageal squamous cell carcinoma tissues was significantly higher than that in normal esophageal tissues. The positive expression rates of BRF2 protein in esophageal squamous cell carcinoma, adjacent esophageal squamous cell carcinoma and normal esophageal tissues were 54.55%, 32.5% and 7.5%, respectively, with a rate in esophageal squamous cell carcinoma and adjacent esophageal squamous cell carcinoma tissues higher than that in normal esophageal tissues (P<0.05). The BRF2 positive expression rate in esophageal squamous cell carcinoma tissues was decreased with the increase in differentiation grade of esophageal squamous cell carcinoma (P<0.05), and the expression rate in grade Ⅲ and Ⅳ of esophageal squamous cell carcinoma significantly higher than that in gradeⅠ and Ⅱ ( 72.7%, 73.3% vs 35.7%, 34.8%， P<0.05). The BRF2 expression rate in the patients who survived less than 3 years was markedly higher than that in the patients who survived longer than 3 years (69.2% vs 38.2%, P<0.05); and smoking patients had a markedly higher expression rate than non-smokers (61.1% vs 30.4%, P<0.05). Conclusion: mRNA and protein of BRF2 gene are highly expressed in esophageal squamous cell carcinoma, which is correlated with poor prognosis of patients, and may be used in evaluating the prognosis of esophageal carcinoma patients.
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Project supported by the National Natural Science Foundation of China (No. 30571844)
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