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Expressions of different Smads gene family members in gastric cardia adenocarcinoma tissues and their clinical significance
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Abstract:
Objective:To investigate the expressions of different Smad genes in gastric cardia adenocarcinoma (GCA) and to explore their correlation and clinical significance. Methods: 110 cases of pathologically confirmed GCA were included from the Fourth Hospital of Hebei Medical University during 2004-2009 year. RT-PCR and immunohistochemistry methods was used to respectively detect the mRNA and protein expressions of Smad2, Smad3, Smad4 and Smad7 in GCA. Their correlations with clinical pathological characteristics of GCA were analyzed. Results: The expressions of Smad2, Smad3 and Smad4 mRNA in GCA tissues were significantly reduced with comparison to the paired normal tissues (\[0.4956±0.1862\] vs \[0.8611±0.2914\], P<0.01; \[0.4713±0.1712\] vs \[0.8314±0.2811\], P<0.01; \[05145±0.1987\] vs \[0.8954±0.2856\], P<0.01), and Smad7 mRNA expression in GCA tissues was significantly increased with comparison to the paired normal tissues (\[0.5114±0.1962\] vs \[0.2012±01006\], P<0.01). The positive protein expression rate of p-Smad2/3 and Smad4 in GCA tissues was significantly lower than that in the paired normal tissues (42.7% vs 93.6%, P<0.01; 45.5% vs 95.5%, P<0.01). The protein expressions of p-Smad2/3 and Smad4 were associated with TNM stage and pathological differentiation (P<0.05). The positive protein expression rate of Smad7 in GCA tissues (48.2%) was significantly higher than that in the paired normal tissues (3.6%) and was associated with pathological differentiation (P<0.05). The protein expression of p-Smad2/3 and Smad4 was positively correlated, while p-Smad2/3 and Smad4 did not show any correlation with Smad7. Conclusion: Decreased expression of Smad2, Smad3 and Smad4 and increased expression of Smad7 in GCA may be associated with the occurrence and development of GCA.
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Project Supported:
Project supported by the Key Project in Medical Scientific Research of Hebei Province (No. 20090465)
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