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Expression and significance of BRAF and EphB2 in human colorectal serrated adenomas
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Abstract:
Objective:To discuss the expression and significance of v-raf murine sarcoma viral oncogene homolog B1(BRAF) and erythropoietin-producing hepatoma cell line B2 (EphB2) in human colorectal serrated adenomas. Methods: Collect 10 paraffin specimens of normal mucosa, 21 cases of hyperplastic polyp, 22 cases of serrated adenomas and 55 cases of traditional colorectal adenomas (18 tubular adenomas, 16 tubulovillous adenomas, 21 villous adenomas) in the Affiliated Hospital of Binzhou Medical College between Jan. 1996 to May. 2008. The expression levels and sites of BRAF and EphB2 protein were examined by immunohistochemical method. Results:Most BRAF protein positive cells in hyperplastic polyp were located in the lower crypt, those in adenomatous polyp located in upper crypt, and those in serrated adenomas almost expressed in the whole crypt. The expression of BRAF protein was similar in serrated adenomas and adenomatous polyp (0.129±0030 vs 0.130±0.026, P>0.05), while more higher than that in hyperplastic polyp (0.129±0.030 vs 0.102±0.014, P<0.01). There was no significant difference in BRAF protein expression among the serrated adenomas, tubular adenomas, tubulovillous adenomas and villous adenomas (0.129±0.030 vs 0.116±0.019, 0.119±0.037, 0.122±0.008, P>0.05). The EphB2 protein positive cells in hyperplastic polyp was almost located in the membrane of the middle and lower region of crypt, those in adenomatous polyp located in the upper crypt, and those in serrated adenomas expressed in the whole crypt. The EphB2 protein expression in serrated adenomas and adenomatous polyp was similar (0.138±0.024 vs 0.139±0.025, P>0.05)，but much higher than that in hyperplastic polyp (0.138±0.024 vs 0.169±0.018, P<0.01). No significant difference was found in EphB2 among serrated adenomas, tubular adenomas, tubulovillous adenomas and villous adenomas (0.138±0.024 vs 0.143±0.027, 0.139±0.028, 0.133±0021, P>0.05). Conclusion: BRAF and EphB2 proteins both in hyperplastic polyp and adenomatous polyp are expressed partly in crypt, while those in colorectal serrated adenomas are expressed in the whole crypt, indicating that serrated adenomas may be an independent tumor difference from adenomatous polyp.
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Project Supported:
Project supported by the Science and Technology Program from Education Bureau of Shandong Province (No. J06L52)
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