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Asymmetric siRNA targeting VEGF inhibits proliferation of breast cancer MCF-7 cells
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Abstract:
Objective: To investigate the inhibitiory effect of siRNA targeting vascular endothelial growth factor (VEGF) gene on proliferation of breast cancer MCF-7 cells. Methods: Small interfering RNA (siRNA) targeting VEGF, including 2 siRNAs (siRNA21/21, siRNA23/23) and 2 asymmetric siRNAs (aiRNA) (aiRNA19/21, aiRNA21/23) were designed and synthesized. The siRNA was transfected into MCF-7 cells, and cell proliferation was detected by MTT method, and the apoptosis was analyzed by flow cytometry. VEGF protein and mRNA expression levels of MCF-7 cells were detected by ELISA and RT-PCR. Results: Compared with the control group, the 4 siRNAs effectively inhibited VEGF mRNA expression (\[714±5.01\]%, \[40.0±3.11\]%, \[37.2±2.79\]%, \[11.1±0.99\]% vs \[2.4±0.11\]%, P<005), and the proliferation of MCF-7 cells (\[44.7±5.38\]%, \[38.5±5.67\]%, \[33.6±2.18\]%, \[33.1±318\]%, \[2.2±0.28\]%, P<0.05), with aiRNA21/23 of the best RNA interference effect. Compared with the control group, the apoptotic rate was significantly increased (\[49.9±4.02\]% vs \[4.7±0.91\]%, P<0.05). Conclusion:siRNA targeting VEGF can inhibit proliferation and induce apoptosis of MCF-7 cells, with asymmetric siRNA showing best effects.
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Project Supported:
Project supported by the National Significant New Drug Creation Project (No. 2009ZX09103-635）
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