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Adenovirus mediated overexpression of miR-99a inhibits the growth of hepato-cellular carcinoma cells
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Abstract:
Objective: To study the inhibitory effect of adenovirus-mediated-miR-99a overexpression on proliferation of hepatocellular carcinoma cells (HCCs) and find a new kind of adenovirus mediated miRNA treatment for cancer.Methods: The expression of miR-99a was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in human liver cell line L-02 and HCC cell lines HepG2, SMMC-7721, Hep3B and Huh7. The type 5 adenovirus vector containing miR-99 (Ad5-miR-99a) was constructed. Huh7 cells were infected with empty adenovirus vector (Ad-blank) or Ad5-miR-99a, and MTT and colony formation assay were used to examine the inhibitory effect of miR-99a on the growth of Huh7 cells. Results: miRNA-99a was markedly decreased in the Huh7 cell line compared with that in L02 cell line (P<0.01). Ad5-miR-99a adenovirus vector was successfully constructed, and miR-99a was stably high expressed in Huh7 cells after infection with Ad5-miR-99a. MTT and colony formation assay showed that Ad5-miR-99a could inhibit the growth and colony formation of Huh7 cells compared with Ad-blank (P<0.01). Conclusion: Adenovirus Ad5-miR-99a is successfully constructed, which can effectively suppress the growth of HCC. Ad5-miR-99a might be a prospective therapeutic approach for HCC in the near future.
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Project supported by the Key Science and Technology Project for the Prevention and Treatment of Major Infectious Diseases of China (No. 2008ZX10002-018)
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