Mobile website
Basic Research
Inhibitory effect of curcumin on proliferation of colon cancer SW480 cells and its mechanism
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To study the effect of curcumin on proliferation and expression ofsurvivin, caspase-3 and p-Akt in colon cancer SW480 cells.Methods:SW480 cells were treated with different concentrations of curcumin (5-40 μmol/L) for 24, 48 and 72 h. The inhibitory effect of curcumin on SW480 cells was detected by MTT. The expressions of survivin, caspase-3 and p-Akt protein were analyzed by Western blotting. Results: Curcumin decreased the proliferation of SW480 cells in a dose- and time-dependent manner (P<0.01), with an inhibitory rate of (75.86±3.93)% after 40 μmol/L curcumin treatment for 72 h. Curcumin decreased the expressions of p-Akt and survivin, and increased the expression of caspase-3 protein in a dose- and time-dependent manner (P<0.01). Treated with curcumin (40 μmol/L) for 48 h, the expressions of p-Akt and survivin protein were decreased significantly (\[0.204±0.025\] vs \[0.367±0.035\], P<0.01; \[0.208±0.014\] vs \[0.385±0.034\], P<0.01), and the expression of caspase-3 protein was increased significantly (\[0.371±0.028\] vs \[0.127±0.023\], P<0.01). Conclusion: Cureumin inhibits the proliferation of SW480 cells, which may be related to a decrease in phosphorylation of Akt, down- regulation of survivin protein expression and up-regulation of caspase-3 protein expression.
Keywords:
Project Supported:
Project supported by Science and Technology Innovation Activities for Undergraduates in Zhejiang Province in 2009
Clc Number:
