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Inhibitory effect of inactivated-haploidentical CTL induced by allogeneic dendritic cells on mouse transplanted lung cancer
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Abstract:
Objective: To investigate the anti-lung cancer effect after infusion of 60Co inactivated-MHC haploidentical cytotoxicity T lymphocytes (CTLs) induced by dendritic cells (DCs). Methods: DCs were cultured from CB6F1 mice and their phenotype was detected by flow cytometry. Mature DC-induced CTLs against metastatic lung cancer cells (Lewis lung cancer, LLC) derived from C57BL/6 mice were cultured in vitro. The LLC tumor bearing C57BL/6 mice were treated with 60Co-inactivated CTL. The anti-lung cancer effect of CTL was identified through cell-mediated cytotoxicity assay. Pathological examination of the liver, spleen, intestinum tenue and skin was performed to observe the graft versus host disease (GVHD) and lung cancer metastasis. The survival time of tumor bearing mice was observed. Results: FCM analysis showed that mature DCs were expanded. The lung weight of LLC metastatic mice treated with inactivated-CTL was decreased obviously (\[0.27±0.06\] vs \[0.52±0.07\] g, P<0.05); and the mean survival time of treated mice prolonged (\[78.10±16.50\] vs \[49.30±6.45\] d, P<0.05). The cell-mediated cytotoxicity of the spleen leukocytes against LLC increased along with the ratio of effector to target (the highest cytotoxicity was \[32.7±1.64\]% when effector〖DK〗∶target =100〖DK〗∶1). There was no obvious GVHD in pathological findings. Conclusion: Inactivated haploidentical CTL can induce antitumor immunological responses and reduce lung cancer metastasis without obvious GVHD.
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Project Supported:
Project supported by Youth Science and Technology Foundation of Shanxi Province (No. 2010021035-6)
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