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G protein-coupled receptor 54 gene expression in osteosarcoma and its clinical significance
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Abstract:
Objective:To explore the relationship between the expression of G protein-coupled receptor (GPR)54 gene and the biologic behavior or prognosis of ostosarcoma patients.Methods: The expression levels of GPR54 mRNA in 3 types of osteosarcoma cell lines (MG-63, Saos-2 and U-2 OS cells) and one normal osteoblast cell line hF-OB 1.19 were examined using RT-PCR. The expression levels of GPR54 protein in 44 osteosarcoma and 12 benign osteochondroma control paraffin specimen sections were detected by immunohistochemistry. Transwell assay was used to detect the migration and invasion abilities of the 3 types of osteosarcoma cell lines. Results: GPR54mRNA was strongly expressed in all the 3 types of osteosarcoma cell lines (MG-63, Saos-2 and U-2 OS) and the normal osteoblast cell line (\[0.87±0.05\] vs \[088±0.07\], \[0.88±0.08\], \[0.86±0.05\], P＞0.05), but no significant difference existed between each other. Transwell assay displayed a gradually decreased aggressive phenomenon in osteosarcoma cell lines MG-63, Saos-2, U-2 OS (\[18.10±3.21\] vs \[32.40±5.36\], \[61.00±7.15\], P<0.05). The positive expression rate of GPR54 was significantly higher in osteosarcoma than in benign osteochondroma control (81.82% vs 41.67%, P<0.05). Significantly shorter average survival time was displayed in the patients with GPR54 protein positive expression than the in those with GPR54 protein negative expression (\[27.39±6.05\] months vs \[40.33±4.88\] months, P<0.05). Conclusion: The positive expression rate of GPR54 gene is higher in osteosarcoma patients than in benign osteochondroma patients, which might be associated with short survival time in osteosarcoma patients.
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Project Supported:
Project supported by the Joint Scientific Research Foundation of Ministry of Health and the Health and Education Bureau of Fujian Province (No.WKJ2008-2-042)
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