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Interferon-α enhances anti-leukemia immunological effect of eosinophils granulocytes
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Abstract:
Objective： To observe the immunological enhancement effect of interferon-α (IFN-α) on anti-leukemia activity of eosinophils granulocytes (EOSs) in patients with chronic myelogenous leukemia (CML) after hydroxycarbmide (OHU) therapy, and to investigate the related immunologic mechanism. Methods: Forty-six BCR-ABL positive CML patients (from Jan. 2010 to Feb. 2012) admitted in the 148th Hospital of PLA were included in this study, and were divided into OHU group (20 cases) and OHC combined IFN-α group (26 cases) with a balance in sex ratio and age range. Meanwhile, specimens of 30 healthy volunteers from the Regular Physical Examination Center of the hospital were collected as control. ELISA was used to determine the concentrations of cytokines IL-6, IL-12, IL-17 and IFN-γ in serum of the CML patients. Cytochemistry staining was used to observe the peroxydase (POX) expression of immunocyte in bone marrow. Immunofluorescence (IF) staining was used to observe cytokines IL-12 and IL-17A expression levels as well as the expression of mannose receptor (MR). Results: The serum concentrations of IL-6, IL-12 and IL-17 were increased significantly in CML patients compared with the healthy control group \[(116.13?15.16) vs (90.98?12.32) pg/ml; (189.26?22.14) vs (96.60?4.92) pg/ml; (34.42?2.16) vs (23.74?1.36) pg/ml, P<0.05\]. After OHU treatment, the serum concentrations of IL-6, IL-12 and IL-17 in the CML patients decreased to (87.14?13.37) , (60.22?20.16) and (17.03?2.16) pg/ml, respectively (P<0.05). Compared with the OHU treatment group, the serum IL-6, IL-12 and IL-17 concentrations in the OHU combined IFN-α treatment group were significantly up-regulated \[(122.04?1025), (101.12?27.16) and (40.16?4.11) pg/ml, P<0.05 or P<0.01\]. EOS in bone marrow of CML patients expressed IL-12, IL-17A and MR. OHU treatment combined with IFN-α could decrease immunosuppressive effect of OHU therapy, the quantity of EOS with positive IL-12, IL-17A and MR expressions was increased obviously, and the POX activity of EOS was also increased. Conclusion: EOS can secrete cytokines IL-12 and IL-17 and express MR. OHU treatment combined with IFN-α can enhance the anti-leukemia immunological effect of EOS and decrease the immunosuppressive effect of OHU therapy on EOS.
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Project Supported:
Project supported by the Major Science and Technology Development Program of “12th Five-year Plan” of Ji’nan Military Area Command (No. JN11LO17)
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