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Mechanism of differentiation of mouse melanoma B16 cells induced by ethanol extract of cochinchina momordica seed
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Abstract:
Objective：To study the mechanism of the differentiation of mouse melanoma B16 cells induced by ethanol extract of cochinchina momordica seed (CMSEE). Methods: After treated with CMSEE, morphological changes of B16 cells were observed by Giemsa staining, and melanin level was assessed by colorimetric method. Western blotting was used to measure the expression changes of phosphorylation p38 (p-p38) and ERK (p-ERK) in B16 cells after treatment with CMSEE and p38, ERK pathway inhibitors, SB203580 and SD98059. Results: CMSEE could induce the differentiation of B16 cells. The melanin level in B16 cells was significantly increased after CMSEE treatment (P<0.01), with D value being (0.057±0.007), (0.173±0.005) and (0.249±0.002) for 10, 20 and 40 μg/ml CMSEE and (0.037±0002) for control group. 20 μg/ml CMSEE elevated the level of p-p38 protein and blocked the expression of p-ERK (P<0.01) in B16 cells, and the expression level of p-p38 protein was 5.6 fold and p-ERK was 0.25 fold that of the control group after treatment with CMSEE for 60 min. SB203580, the inhibitor of p38 pathway, blocked CMSEE-induced B16 cell differentiation, and PD98059, the inhibitor of ERK pathway, elevated CMSEE-induced B16 cell differentiation. Conclusion: p38 and ERK signal pathway activations are involved in the differentiation of B16 melanoma cells induced by CMSEE.
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Project Supported:
Project supported by the Foundation of Administration of Traditional Chinese Medicine in Hebei Province (No. 2011011)
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