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Krüppel-like factors 4 modulates the effects of chemotherapy and photodynamic therapy on hepatocacinoma HepG2 cells
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Abstract:
Objective To explore the regulatory effects of Krüppel-like factors 4 (KLF4) on chemotherapy and photodynamic therapy on HepG2 cells. Methods: Lentiviral pWPTS-KLF4 vector containing human KLF4 was constructed. The expression of KLF4 mRNA and protein in HepG2 cells after pWPTS-KLF4 infection was analyzed by RT-PCR and Western blotting; the tolerance of HepG2 cells to cisplatin, cyclophosphamide and fluorouracil and the sensitivity of HepG2 cells to photodynamic therapy were evaluated by MTT assay; the change of mitochondrial membrane potential in HepG2 cells was examined by Rhodamine 123 staining. Results: Lentiviral pWPTS-KLF4 vector was successfully constructed. Treated with cisplatin, cyclophosphamide or fluorouracil for 72 h, the vialble HepG2 cells were significantly increased after pWPTS-KLF4 infection (\[43.43±4.78\]% vs \[18.09±1.02\]%；\[110.51±4.58\]% vs \[75.23±592\]%；\[34.55±293\]% vs \[19.16±1.32\]%, P<0.01); Treated with ALA mediated photodynamic therapy for 24 h, vialble HepG2 cells were significantly decreased after pWPTS-KLF4 infection (\[37.16±3.26\]% vs \[57.24±801\]%, P<0.01), and mitochondrial membrane potential was significantly decreased. Conclusion: Recombinant human KLF4 can increase the tolerance of HepG2 cells to chemotherapy and the sensitivity of HepG2 cells to photodynamic therapy.
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Project supported by the Natural Science Foundation of Tianjin (No. 10JCYBJC1150)
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