Silencing Rac1gene expression via RNA interference affects cytoskeleton and cell cycle of colorectal cancer LoVo cells
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Abstract:
Objective:To detect the expression of Rac1 protein in colorectal cancer cells and to observe its correlation with cytoskeleton, cell cycle and apoptosis of colorectal cancer LoVo cells. Methods: The expression of Rac1 protein was detected by Western blotting in five colorectal cancer cell lines. The cytoskeleton changes were observed by confocal microscopy in LoVo cells transfected with Rac1-shRNA. The cell cycle and apoptosis were observed by flow cytometry in LoVo cells transfected with Rac1-shRNA. Results: Rac1 protein was highly expressed in five colorectal cancer cells. The cross-linked F-actin network was significantly reduced and disordered in LoVo cells transfected with Rac1-shRNA. The cell number in G0/G1 phase was significantly increased in Rac1-shRNA group compared with the control group (\[74.63±440\]% vs \[56.46±3.09\]%,P<0.05), whereas it was reduced in S phase (\[12.87±1.77\]% vs \[29.66±192\]%,P<0.05) and the apoptosis rate of LoVo cells was significantly increased in Rac1-shRNA group compared with the control group (\[25.31±2.05\]% vs \[9.38±1.16\]%, P<0.05). Conclusion: Silencing Rac1 expression mediated by RNA interference affects the formation of cytoskeleton and the cell cycle of LoVo cells, which provides clinical evidence for Rac1-targeted treatment of colorectal cancer.
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Project supported by the Science and Technology Foundation of Ji′nan Science and Technology Bureau (No. 2007-153), and the Science and Technology Foundation of Ji′nan Health Bureau (No. 2008-089)