Inhibitory effect of recombinant adenovirus Ad-CRT/MAGE-A3 combined with epirubicin on proliferation and invasion of breast cancer cells

doi:10.3872/j.issn.1007-385X.2012.5.005

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Archive > Volume 19 Issue 5 > 2012,19(5):491-495. DOI:10.3872/j.issn.1007-385X.2012.5.005 Prev Next

Basic Research

Inhibitory effect of recombinant adenovirus Ad-CRT/MAGE-A3 combined with epirubicin on proliferation and invasion of breast cancer cells

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Abstract:

Objective : To construct a recombinant adenovirus for calreticulin (CRT)/melanoma antigen gene-A3 (MAGE-A3) and evaluate its inhibitory effect on breast cancer MDA-MB-231 cells. Methods: Ad-CRT/MAGE-A3 vector was constructed and infected into MDA-MB-231 cells. The optimal multiplicity of infection (MOI) was obtained by fluorescence microscope method. There were five groups: epirubicin, Ad-GFP, Ad-CRT/MAGE-A3, epirubicin+Ad-GFP and epirubicin+Ad-CRT/MAGE-A3. The proliferation and invasion of MDA-MB-231 cells in different groups were detected by MTT and Transwell assays. Results: Recombinant adenovirus vector Ad-CRT/MAGE-A3 was constructed successfully, and its optimal MOI in MDA-MB-231 cells was 100. Ad-CRT/MAGE-A3 combined with epirubicin decreased the proliferation (\[83.27±1.04\]% vs \[57.42±1.27\]%, \[43.26±0.95\]%, \[61.23±1.47\]%, \[55.38±1.62\]%, P<0.05) and the invasion (\[8.41±4.20\] vs \[14.62±5.33\], \[107.66±3.35\], \[100.60±442\], \[104.20±260\], P<0.05) of MDA-MB-231 cells significantly. Conclusion: Recombinant adenovirus vector Ad-CRT/MAGE-A3 can enhance epirubicin to inhibit the proliferation and invasion of breast cancer MDA-MB-231 cells.

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Project supported by the Natural Science Foundation of Liaoning Province (No. 20042073), and the Science and Technology Foundation of Liaoning Province (No. 2009225008-1)

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