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Effect of triterpenes compound of cortex periplocae on PCNA expression in rat esophageal carcinoma
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Abstract:
Objective : To investigate the effect of triterpenes compound of cortex periplocae (TCCP) on the expression of proliferating cell nuclear antigen (PCNA) in N-nitrosomethylbenzylamine (NMBA)-induced rat esophageal carcinoma. Methods: 120 male F344 rats were randomly divided into four groups: a model group treated with NMBA only and a TCCP treatment group receiving NMBA plus TCCP, a soya oil control group treated with soya oil and a normal control group. At 9, 15 and 25 weeks after treatment, the pathological changes of esophageal tissues were detected by H-E staining, while the expression of PCNA was measured by immunohistochemistry. Results: There were no abnormal changes in the normal and soya oil groups. At 9, 15 and 25 weeks after treatment, the incidence of preneoplastic lesion in the NMBA group was 20.0%, 46.7% and 933%, respectively. At 9 and 15 weeks, the rate of rat esophageal preneoplastic lesion significantly decreased in TCCP treatment group compared with NMBA group (0,0 vs 20.0%, 46.7%, P<0.05). There was a significant increase in the expression of PCNA in the NMBA group (week 9: 213.17±29.74; week 15: 268.35±39.56; week 25: 327.24±28.19), compared with that in the normal control group (\[167.96±2016\], \[170.76±14.79\], \[172.49±17.49\], P<0.05). TCCP significantly decreased the expression of PCNA compared with that in the NMBA group (P<0.05). Conclusion: TCCP inhibits NMBA-induced rat esophageal carcinoma probably via suppression of PCNA expression.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30772752), and the Natural Science Foundation of Hebei Province (No.C2008000952)
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