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Effect of IL-21 on regulatory T cells in chronic myeloid leukemia patients
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Abstract:
Objective : To obverse the effect of interleukin-21(IL-21) on CD4+CD25+ regulatory T cells (Tregs) in chronic myeloid leukemia (CML) patients. Methods: Peripheral blood mononuclear cells (PBMCs) were collected from newly diagnosed CML patients in the Hematology Department of Second Hospital of Lanzhou University, and CD4+T cells were isolated by magnetic beads. CD4+T cells were co-cultured with IL-21 (50, 100, and 200 ng/ml) and saline respectively for 72 h, and then the Treg ratio was determined by flow cytometry; Foxp3 mRNA expression was examined by real-time PCR; and IL-10 and TGF-β in different cell supernatants were detected by ELISA. Results: Compared with the control group, the ratios of Treg in CD4+T cells were decreased in 50 ng/ml, 100 ng/ml and 200 ng/ml IL-21 groups (\[342±0.76\]%, \[6.81±0.33\]%, \[7.98±0.76\]% vs \[12.09±0.91\]%， P<0.05); and Foxp3 mRNA expressions were also decreased (\[0.05±0.02\], \[0.16±002\], \[0.25±0.02\] vs 1, P<0.05), the levels of IL-10 (\[2613±7.28\], \[44.88±3.72\], \[79.77±394\] vs \[133.00±12.32\] pg/ml, P<0.05) and TGF-β (\[9.25±0.84\], \[16.70±1.00\], \[20.47±1.59\] vs \[26.05±1.81\] pg/ml, P<0.05) in cell supernatants were significantly decreased. Conclusion: IL-21 can inhibit the ratio and function of Treg in peripheral blood of CML patients.
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Project Supported:
Project supported by the Science and Technology Supporting and Social Development Program of Gansu Province (No.0804NKCA115)
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