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Expression of γ-synuclein in esophageal carcinoma and its effect on invasion of esophageal carcinoma Eca-109 cells
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Abstract:
Objective : To detect the expression of γ-synuclein in esophageal adenocarcinoma and evaluate its effect on invasion of esophageal carcinoma Eca-109 cells. Methods: SP immunohistochemical staining was used to examine the expression of γ-synuclein in 30 esophageal carcinoma specimens and 10 normal control specimens from January to December 2010 (Fourth Hospital of Hebei Medical University). The plasmid containing γ-synuclein (pcDNA3.0-γ-synuclein) was transfected into Eca-109 cells by lipofectamine. The expressions of γ-synuclein mRNA and protein were investigated by RT-PCR and flow cytometry, respectively. The invasion ability of transfected Eca-109 cells was dectected by Transwell assay. Results: The positive exppression rate of γ-synuclein was 83% in human esophageal carcinoma, which was significantly higher than that in normal esophageal tissues (P<0.05), and the positive rate of γ-synuclein in stage-Ⅱ esophageal carcinoma patients was significantly lower than that in stage Ⅲ-Ⅳ patients (22.2% vs 76.2%，P<0.05). By comparing transfected group in Eca-109 cells with empty plasmid group and control group, RT-PCR showed the expression level of γ-synuclein mRNA increased significantly (\[1.10±0.03\] vs \[0.42±0.03\]，\[0.45±0.15\]; P<0.01). Flow cytometry showed that the expression level of γ-synuclein protein increased significantly compared with the other two groups (\[1.05±0.06\] vs \[0.80±0.45\], \[0.79±0.46\]; P<0.05). Invasion through matrigel abilities of Eca-109 cells increased significantly (\[167±2.51\] vs \[65±2.60\], \[70±2.50\]; P<0.01). Conclusion: γ-synuclein is highly expressed in esophageal carcinoma tissues and can enhance the invasion of Eca-109 cells, which may play an important role in carcinogenesis progression and metastasis of esophageal carcinoma.
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Project Supported:
Project supported by the Natural Science Foundation of Hebei Provience (No. C2008000948)
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