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Effects of miRNA-34a on proliferation and apoptosis of glioma SHG-44 cells
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Abstract:
Objective:To investigate the expression levl of microRNA-34a (miR-34a) in human glioma tissues, and further explore the role of miR-34a on proliferation and apoptosis of glioma SHG-44 cells. Methods: Twenty glioma samples were collected from the Department of Neurosurgery, Affiliated Hospital of Qingdao Medical College (January 2007 to December 2010). The normal brain tissues were obtained from 5 patients with severe traumatic brain injury who required post-trauma surgery. The expression level of MiR-34a in glioma tissues was detected by real-time PCR. After transfection of miR-34a mimics into SHG-44 cells, the proliferation, cell cycle and apoptosis were measured by MTT and flow cytometry, respectively. Results: The expression level of miR-34a was lower in the glioma tissues compared with the normal brain tissues, and miR-34a expression level was lower in the glima tissues of phase Ⅲ/Ⅳ than in phase Ⅰ/Ⅱ. The cell proliferation inhibitory rate increased signficantly in the miR-34a transfected group compared to the blank group (\[37.24±572\] % vs \[4.19±063\]%, P<0.01), the ratio of cells arrest at G1 phase was significantly higher in the miR-34a mimics transfected group compared to the blank group (\[61.78±2.01\]% vs \[50.91±1.19\]%, P<0.05), and the cell apoptosis in the miR-34a transfected group was significantly increased compared to the blank group (\[15.28±365\]% vs \[2.07±0.84\]%, P<001). Conclusion: MiR-34a was lowly expressed in human glioma tissues. MiR-34a can inhibit SHG-44 cell proliferation, thus inducing SHG-44 cell cycle arrest and promoting SHG-44 cell apoptosis.
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Project supported by the Key Program of Natural Science Foundation of Shandong Province (No.Y2006C02)
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