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Inhibitory effect of umbilical cord blood mesenchymal stem cells infected with reconstructed lentivirus-TNF-α on growth of gastric cancer transplantation tumors
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Abstract:
Objective:To study the inhibitory effect of umbilical cord blood mesenchymal stem cells (UCBMSCs) infected with reconstructed lentivirus-TNF-α on growth of gastric cancer transplantation tumors, based on UCBMSC as a TNF-α carrier. Methods: The human gastric cancer SGC-7901 cells were injected into nude mice subcutaneously groin. The model of transplanted SGC-7901 cells in nude mice was set up. The reconstructed lentivirus (Lv-TNF-α) and empty lentivirus (Lv-EGFP) were added to UCBMSC, and UCBMSC-TNF-α cells and control UCBMSC-EGFP cells were obtained. Tumor-bearing nude mice were separated into three groups randomly: a UCBMSC-TNF-α group, an UCBMSC-EGFP group and a NaCl group. The nude mice in these three groups were injected around the tumor with UCBMSC-TNF-α cells, UCBMSC-EGFP cells or NaCl. The transplanted gastric cancer volume and weight was observed; the expressions of TNF-α mRNA and protein in the three groups were determined by RT-PCR and ELISA; and the necrosis areas in the tumors were observed by H-E staining. Results: The transplantation tumor model was established in the nude mice successfully. The transplanted tumor average volume in the three groups were (0.51±0.27), (0.64±0.36) and (1.21±0.80) cm3, and the transplanted tumor average volume in the UCBMSC-TNF-α group was minimum (F=3.88，P<005); The expressions of TNF-α mRNA in the three groups were (1.92±0.12), (1.21±0.26) and (0.81±0.22), and the expression of TNF-α mRNA in the UCBMSC-TNF-α group was maximum (F=54.82, P<0.01); The expressions of TNF-α protein in the three groups were (148.29±3.76), (78.22±6.24) and (42.80±3.02) pg/ml, and the expression of TNF-α protein in the UCBMSC-TNF-α group was maximum (F=694.54, P<0.01); H-E stained biopsy revealed that the largest tumor necrosis was in the UCBMSC-TNF-α treatment group. Conclusion: Transgenic UCBMSCs can paracrine TNF-α, which has an inhibitory effect on gastric cancer.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30772542)
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