Mobile website
Clinical Research
In vitro expansion of autologous NK cells from renal cell carcinoma patients and its cytotoxicity against human rencal cell carcinoma 786-O cells
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To explore the expansion method of autologous NK cells from renal cell carcinoma (RCC) patients based on IL-15- and 4-1BBL-modified K562 cells (modi-K562 cells) combined with IL-2 and to investigate their cytotoxicity against RCC 786-O cell lines. Methods: Peripheral blood mononuclear cells (PBMCs) from 10 RCC patients were co-cultured with modi-K562 cells in medium containing various concentrations of IL-2 for 14 d. The expansion, immune phenotype and cytotoxicity against 786-O cells were analyzed by flow cytometry and Calcein-AM release assay. Results: modi-K562 combined with IL-2 effectively amplified NK cells in vitro. The number of NK cells from 300 IU/mL IL-2 co-culture system for 14 d had amplified on an average of （202.4±12.8) fold. Moreover, the expanded NK cells significantly increased cytotoxicity against 786-O cells. At an effect/target (E∶T) ratio of 20∶1, (72.0±4.3)% of the targets were killed by the expanded NK cells, whereas unexpanded ones killed only (34.2±3.6)% of targets (P<0.01). Conclusion: modi-K562 cells combined with IL-2 can effectively stimulate the expansion of RCC patients’ NK cells in vitro, which significantly augment the cytotoxicity against RCC 786-O cells.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China (No.81071856, No.30973450), the Shanghai Pujiang Program (No.11PJ1407900), and the Shanghai Tenth People’s Hospital Foundation (No.10RD103, No.11SC103)
Clc Number:
