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Archive > Volume 24 Issue 12 > 2017,24(12):1381-1385. DOI:10.3872/j.issn.1007-385X.2017.12.007 Prev Next
Basic Research
MGBA carrying umbilical cord blood-derived dendritic cells induces specific cytotoxic T lymphocytes against breast cancer cells
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Abstract:
Objective: To investigate the in vitro cytotoxicity of cytotoxic T lymphocytes (CTLs) induced from umbilical cord blood derived dendritic cells (DCs) that sensitized by mammaglobin A (MGBA) (MGBAp-DCs) against breast cancer. Methods: Umbilical cord blood (UCB) samples were obtained from healthy pregnant women during cesarean delivery and used to isolate UCB mononuclear cells to induce DC. MGBAp-DCs were co-cultured with autologous lymphocytes to induce CTL. The DC phenotypes (CD83, CD86, HLA-DR) were determined by using flow cytometry; and IL-10, IL-12 secretion levels was determined by ELISA; the killing effect of CTL against breast cancer cells was detected by CCK-8. Results: Mature DCs were successfully isolated with typical morphology and functionality characteristics. Specific CTL induced by MGBAp- DCs had a significant cytotoxic effect against breast cancer MDA-MB-415 cells (P<0.05). Cytotoxic effect was significantly weakened after adding anti-HLA-I antibody, but not with adding anti-HLA-II antibody. However, the the cytotoxicity of specific CTL on normal breast cell line MCF-10 was not influenced by the presence of anti-HLA-I or anti-HLA-II antibodies or not. Conclusion:MGBAp-DCs can effectively enhance the cytotocxity of CTL against breast cancer cells in vitro.
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Project Supported:
Project supported by the Science and Technology Project of Liaoning Province (No.2011415052-3)
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