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miRNA-100 promotes hepatic carcinoma HepG2 cell apoptosis through down-regulating polo-like kinase 1 expression
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Abstract:
Objective:To explore the effects of microRNA-100（miR-100）on expression of polo-like kinase 1 (Plk1) and the apoptosis of human hepatic carcinoma HepG2 cells. Methods: HepG2 cells were transfected with miR-100 mimics by oligofectamine. RT-PCR and immunofluorescence were used to analyze Plk1 mRNA and protein expressions in HepG2 cells, respectively. Moreover, the apoptosis of HepG2 cells was detected by AnnexinⅤ-FITC kit. Results: HepG2 cells were successfully transfected by miR-100 mimics and the transfection efficiency was (88.75±2.22) %. 48 h after transfection, the expression of Plk1 mRNA decreased significantly in the miR-100 mimics transfection group compared with the negative control, blank control, and liposome groups (\[0.71±0.01\] vs \[0.95±0.01\], \[0.92±0.02\], \[0.93±002\]，P<0.01). 72 h after transfection, Plk1 protein expression was almost undetectable in HepG2 cells transfected with miR-100 mimics. Meanwhile, the cell apoptosis rate in the miR-100 mimics group was significantly increased in comparison with those in the negative control, blank control, and liposome groups (\[26.95±6.72\]% vs \[15.03±512\]%, \[6.88±3.71\]%, \[9.00±3.37\]%, P<0.05). Conclusion: miR-100 can inhibit the expression of Plk1 gene, therefore promoting the apoptosis of hepatic carcinoma HepG2 cells.
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Project Supported:
Project supported by the Science Foundation for Distinguished Young Scholars of He’nan Province （No. 0512000800）
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