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Adenovirus-mediated over-expression of PTEN inhibits the growth of human glioma U251 cell xenografts in nude mice
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Abstract:
Objective:To investigate the inhibitory effect of recombinant adenovirus (Ad-PTEN-GFP) containing phosphatase and tensin homologue deleted on chromosome 10 (PTEN) gene on the growth of human glioma U251 cell xenografts in nude mice. Methods: Human glioma U251 cells were injected under the skin of the dorsum to establish a nude mouse glioma model. The tumor-bearing nude mice were randomly divided into three groups: Ad-PTEN-GFP group，Ad-GFP group (empty vector group) and PBS group (blank group).The growth of tumor xenografts in nude mice of the three groups was observed. The tumor volumes were measured, the tumor growth curves were drawn and the survival time of the nude mice bearing tumors was observed. The cell apoptosis of tumor cells was detected by TUNEL assay, and the expressions of PTEN and P65 proteins in tumor tissues were detected by immunohistochemical assay. Results: Compared with the Ad-GFP group, the growth of glioma cell xenografts in the Ad-PTEN-GFP group was inhibited with tumor volume inhibition rate increasing significantly (\[82.5±12.7\]% vs \[72±1.3\]%, P<0.05), and the survival time of tumor-bearing nude mice was prolonged (\[103±10\] vs \[58±8\] d, P<0.01\]. TUNEL assay results showed that the apoptotic rate of glioma cells was significantly increased (\[46.4±8.3\]% vs \[4.6±1.0\]%, P<0.01\]. Immunohistochemical assay results showed that the positive expression rate of PTEN protein was increased in the Ad-PTEN-GFP group compared with the Ad-GFP group (83.3% vs 0, P<0.01), and the positive expression rate of P65 protein was decreased (16.7% vs 66.7%, P<0.01). Conclusion: The growth of human glioma U251 cell xenografts in nude mice is suppressed after Ad-PTEN-GFP infection, which may be related with the down-regulation of P65 protein expression.
Keywords:
glioma ; U251cell ; PTEN ; P65 ; apoptosis ; recombinant adenovirus vector
Project Supported:
Project supported by the Natural Science Foundation of Hebei Province (No. 2012201136), and the Medical Science Special Foundation of Hebei University (No. 2012B2004)
