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EGFR protein expression and EGFR gene mutation in endometrial carcinoma
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Abstract:
Objective:To study the expression and gene mutation of epidermal growth factor receptor (EGFR) in endometrial carcinoma tissues. Methods: One hundred and four paraffin-embedded endometrial tissues were obtained from Tumor Hospital of Jiangxi Province and the Second Affiliated Hospital of Nanchang University from January 2007 to April 2011, including 56 endometrial carcinoma tissues, 18 endometrial atypical hyperplasia tissues, and 30 normal endometrial tissues. Immunohistochemistry was used to detect the expression of EGFR protein in the above tissues. Exon 19 and exon 21 of EGFR gene in endometrial carcinoma or normal endometrial tissues were amplified by PCR assay, and the mutations were detected by sequencing. Results: The positive expression rate of EGFR in the endometrial carcinoma tissues was higher than that in the normal endometrial tissues (73.2% \[41/56\] vs 30.0% \[9/30\], P<0.01) and that in atypical hyperplasia tissues (73.2% \[41/56\] vs 44.4% \[8/18\], P<0.05). Further analysis indicated that, the positive expression rate of EGFR in the G3 endometrial carcinoma tissues was significantly higher than that in G1 (81.8% \[9/11\] vs 66.7% \[12/18\], P<0.01) and G2 tissues (81.8% \[9/11\] vs 74.1% \[20/27\], P<0.05), and the positive expression rate of EGFR in the >1/2 myometrial invasion group was higher than that in the ≤1/2 myometrial invasion group (86.8% \[33/38\] vs 44.4% \[8/18\], P<0.01). However, the expression of EGFR had no correlation with the FIGO stage and the lymph node metastasis of endometrial carcinoma. One endometrial carcinoma case showed the mutation of exon 19 (G2281A) in EGFR gene, whereas, no mutation was found in exon 21. Conclusion: The positive expression rate of EGFR in endometrial carcinoma is correlated with the histological grade and the infiltration depth of muscular layer, and some endometrial carcinoma tissues show the mutation of exon 19 (G2281A) in EGFR gene.
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