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Archive > Volume 20 Issue 3 > 2013,20(3):284-288. DOI:10.3872/j.issn.1007-385X.2013.03.005 Prev Next
Basic Research
Interleukin-22 promotes proliferation of colorectal cancer cells via STAT3 signaling activation and its possible mechanism
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Abstract:
Objective:To explore the effect of IL-22 on the proliferation of colorectal cancer cells and to illustrate its underlying molecular mechanism. Methods: The relative expression levels of IL-22 receptor 1 ( IL-22R1 ) mRNA in colorectal cancer SW480 and SW620 cells were detected by real-time PCR. The different mass concentrations of IL-22 (0, 1, 5, 10 ng/ml) were used to treat colorectal cancer cells. The effect of IL-22 on the proliferation of SW480 and SW620 cells was analyzed by MTT assay and colony formation assay. SW480 and SW620 cells were stimulated with IL-22 for various time points and were subjected to Western blotting for the detection of protein phosphorylations, including STAT3, AKT, ERK, JNK and P38. Whether the specific inhibitor LLL12 blocking phosphorylation of STAT3 could promote the proliferation of colorectal cancer cells induced by IL-22 was observed. Results: IL-22R1 mRNA was expressed in colorectal cancer cells (SW480, SW620). IL-22 can promote the proliferation of SW480 and SW620 cells in a dose-dependent manner. After the treatment with IL-22 (10 ng/ml), the cell proliferation folds were significantly increased (SW480: \[5.18±0.212\] vs \[2.64±0.27\], SW620: \[814±0.61\] vs \[6.08±0.096\], P<0.01). Moreover, IL-22 enanced the colony formation ability of SW480 and SW620 cells. The colony numbers of IL-22 treating group were significantly higher compared with the control group (\[1 680.67±124.05\] vs \[730±64.29\], \[2 668±116.37\] vs \[1 294±171.61\], P<0.01). Western blotting demonstrated that STAT3 pathway can be activated after the treatment of IL-22. However, there was no significant alteration in AKT, ERK, JNK, P38 pathways. The proliferative effect of IL-22 can be attenuated after the treatment of STAT3 phosphorylation inhibitor LLL12. Conclusion: IL-22 may promote the proliferation of colon cancer SW480 and SW620 cells via STAT3 signaling pathway.
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Project Supported:
Project supported by the Basic Research Foundation from Science and Technology Commission of Shanghai (No.10DJ1400504), and the Biomedical Engineering Crossover Foundation of Shanghai Jiao Tong University (No.YG2011MS32)
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