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Archive > Volume 20 Issue 3 > 2013,20(3):301-305. DOI:10.3872/j.issn.1007-385X.2013.03.008 Prev Next
Basic Research
Silencing KLF4 expression by siRNA promotes proliferation and migration of esophageal cancer KYSE140 cells
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Abstract:
Objective:To investigate the effect of in vitro silencing Kruppel like factor 4 ( KLF4 ) gene expression on the proliferation and migration of esophageal cancer KYSE140 cells. Methods: Western blotting was used to detect the expression of KLF4 protein in the esophageal cancer cell lines, including KYSE140, KYSE150, EC109 and EC9706，and immortalized esophageal NE3 cells. Two pairs of siRNAs targeting KLF4 (KLF4-siRNA1，KLF4-siRNA2) and control siRNA (Ctrl-siRNA) were chemically synthesized, and were transfected into KYSE140 cells with a high expression of KLF4 in vitro to form KLF4-siRNA1-KYSE140, KLF4-siRNA2-KYSE140 and Ctrl-siRNA-KYSE140 cells. The proliferation and migration of esophageal cancer KYSE140 cells after transfection were detected by MTT assay and Transwell assay, respectively. Results: The expression of KLF4 in KYSE140 cells was higher than that in KYSE150, EC109 and EC9706 cells (\[562±0.02\] vs \[171±0.23\], \[3.24±0.35\], \[3.16±0.41\], both P<0.05). Compared with that in Ctrl-siRNA-KYSE140 cells, the expression of KLF4 protein was significantly decreased in KLF4-siRNA1-KYSE140 and KLF4-siRNA2-KYSE140 cells (\[0.49±0.18\], \[0.32±0.09\] vs \[0.98±0.19\], both P＜0.05), the capacities of proliferation were significantly increased (\[12±0.8\], \[1.4±0.1\] vs \[0.6±0.1\], both P<0.05), and the numbers of migration were also significantly increased (\[780±22\], \[475±25\] vs \[83±17\], P<0.05). Conclusion:KLF4 functions as a negative regulator in the proliferation and migration of esophageal cancer cells.
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Project Supported:
Project supported by the Natural Science Foundation of Guangdong Province (No. 10451008901005515, No. 10151008901000177)
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