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Archive > Volume 20 Issue 4 > 2013,20(4):404-408. DOI:10.3872/j.issn.1007-385X.2013.04.004 Prev Next
Basic Research
Dendritic cells induced by IFN-α combined with GM-CSF from peripheral blood mononuclear cells of gastric cancer patients
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Abstract:
Objective: To investigate the possibility of inducing dendritic cells (DCs) by interferon-α (IFN-α) combined with granulocyte-macrophage colony-stimulating factor (GM-CSF) from peripheral blood mononuclear cells (PBMCs) in gastric cancer patients. Methods: PBMCs from 10 gastric cancer patients were cultivated using granulocyte macrophage colony stimulating factor (GM-CSF) 100 ng/ml combined with IFN-α 500 IU/ml (named IFN-α DC) or IL-4 50 ng/ml (named IL-4 DCs) and then CD40L and LPS were added to induce DC maturation. The morphologic features of IFN-α DCs and IL-4 DCs were observed by Giemsa staining. The expressions of CD1a, CD80, CD83, CD86 and HLA-DR on the surface of IFN-α DCs and IL-4 DCs were assayed by flow cytometry. The abilities of IFN-α DCs and IL-4 DCs to induce the proliferation of allogenic T cells were determined by mixed lymphocyte reaction (MLR). Results: Both IFN-α DCs and IL-4 DCs displayed typical DC features in morphology. The expressions of CD1a, CD80, CD83, CD86 and HLA-DR in IFN-α DCs and IL-4 DCs were achieved at high levels at 3 d and 5 d after induced. Mature IFN-α DCs expressed a higher value of CD83 (\[78.25±15.36\]% vs \[50.14±10.24\]%, P<0.05) and CD86 (\[84.84±1012\]% vs \[62.93±15.12\]%, P<0.05) than mature IL-4 DCs. Mature IFN-α DCs was stronger than immature IFN-α DCs on the ability to induce proliferation of allogenic T cells (P<0.05). At the ratios of DCs〖DK〗∶T cell being 1∶40 and 1∶20, mature IFN-α DCs had a stronger ability to induce proliferation of allogeneic T cells than did mature IL-4 DCs (\[39.43±9.21\]% vs \[27.34±10.63\]%, P<0.05; \[60.31±7.86\]% vs \[48.63±625\]%, P<0.05). Conclusion: IFN-α combined with GM-CSF can induce the differentiation of DCs from PBMCs of gastric cancer patients, which have a shorter culture period and stronger ability to induce the proliferation of allogenic T cells than traditional DCs induced by IL-4 and GM-CSF. It may result from the up-regulation of CD83 and CD86 expressions on IFN-α DCs.
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Project Supported:
Project supported by the International Cooperation Projects of Science and Technology Bureau of Jilin Province（No. 20100749）, and the Major Scientific Research Projects of Double Tenth Engineering of Science and Technology Bureau of Jinlin Province (No. 11ZDGG003)
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