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Archive > Volume 20 Issue 4 > 2013,20(4):409-413. DOI:10.3872/j.issn.1007-385X.2013.04.005 Prev Next
Basic Research
Inhibitory effects of recombinant adenovirus- p53 on human lung adenocarcinoma H1299 cells in vitro and in vivo
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Abstract:
Objective: To investigate the inhibitoty effects of recombinant adenovirus-p53 (rAd-p53) on the growth of lung adenocarcinoma H1299 cells (wtP53-/-) in vitro and in vivo, and observe the treatment feasibility of lung adenocarcinoma with tail intravenous injection of rAd-p53. Methods: MTT assay was performed to detect the inhibitory effect of rAd-p53 on the proliferation of H1299 cells. After transfected by rAd-p53 with multiplicity of infection (MOI)=500, the expression of p53 mRNA in H1299 cells was detected by RT-PCR at 24 h; the expression of P53 protein in H1299 cells and the apoptosis of H1299 cells were detected at 72 h by Western blotting and flow cytometry, respectively. BALB/c nude mice were injected subcutaneously with H1299 cells to establish a lung adenocarcinoma nude mice model and then the mice were intravenously administrated by rAd-p53; the tumor growth was observed and tumor growth curve was drawn. Results: H1299 cells were infected by rAd-p53with MOI=500; after infection for 24 h, wild-type p53mRNA was expressed in rAd-p53 group, and at 72 h, wt P53 protein was detected in rAd-p53group. rAd-p53infection could significantly inhibit the proliferation of H1299 cells, the cell proliferation ratio of rAd-p53 group was significant lower than that of the control group (2.8±0.4 vs 6.1±0.5, P<005). The apoptotic rates of H1299 cells in rAd-p53group were increased with time, which were significantly higher than those in the control group (\[27.6±0.05\]% vs \[4.9±0.09\]%, P＜0.01) after infection for 48 h. H1299 tumor-bearing nude mice were successfully established, and the tumor volume of rAd-p53 group was significantly smaller than that of the control group even two weeks after tail intravenous injection (\[0.875±0253\] cm3 vs \[0.479±0.215\] cm3,P<0.05). Conclusion: Tail intravenous infection of rAd-p53 could up-regulate the protein expression of P53 in H1299 cells, then restrain the growth of H1299 cells, promote the apoptosis and significantly inhibit the growth of H1299 cell xenograft tumors in nude mice.
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Project supported by the Science and Technology Program of Shaanxi Province （No. 2010k14-02-01）
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