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Archive > Volume 20 Issue 4 > 2013,20(4):414-418. DOI:10.3872/j.issn.1007-385X.2013.04.006 Prev Next
Basic Research
Lentivirus-mediated NDRG2 overexpression inhibits invasion and metastasis of human bladder cancer T24 cells
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Abstract:
Objective: To observe the effect of NDRG2 (N-myc downstream-regulated gene 2) overexpression on invasion and metastasis of human bladder cancer T24 cell in vitro. Methods: The T24 cells were infected with lentivirus carrying NDRG2 gene (Lenti-NDRG2) to construct a stable cell line overexpression NDRG2.Western blotting was performed to examine the expression of NDRG2, matrix metalloproteinase (MMP)-2 and MMP-9 in T24 cells. The effects of NDRG2 overexpression on the invasion and migration of T24 cells were examined by the transwell invasion and migration assays, respectively. Results: The T24 cell line stable overexpression NDRG2was successfully constructed. Compared with the LEN-LacZ group and the control group, the NDRG2 expression was efficiently increased in the Lenti-NDRG2 group (\[301±1.27\] vs \[9.9±1.24\], \[8.2±1.28\]; P<0.01); the MMP-2 (\[13.5±1.32\] vs \[30.7±1.29\], \[28.8±1.30\]; P<0.01) and MMP-9 expressions (\[11.7±1.27\] vs \[25.2±1.28\], \[26.4±1.31\]; P<0.01) in T24 cells of Lenti-NDRG2 group were significantly suppressed; the capabilities of the invasion (\[18.1±3.4\] vs \[88.5±4.2\], \[90.2±4.1\]; P<0.01）and migration (\[20.1±3.5\] vs \[109.4±5.6\], \[113.0±4.9\]; P<0.01) of T24 cells were significantly suppressed in the Lenti-NDRG2 group. Conclusion: The lentivirus-mediated NDRG2 overexpression can suppress the invasion and migration of human bladder cancer T24 cells.
Keywords:
bladder cancer ; T24 cell ; N-Myc downstream-regulated gene 2( NDRG2 ) ; invasion ; metastasis
Project Supported:
Project supported by the National Natural Science Foundation of China (No.31070681)
