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Archive > Volume 20 Issue 4 > 2013,20(4):444-448. DOI:10.3872/j.issn.1007-385X.2013.04.011 Prev Next
Basic Research
Inhibitory effect of sachalin rhodiola rhizome extract on CD4+CD25+ regulatory T cells in xenograft tumors of Lewis lung cancer bearing mice
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Abstract:
Objective: To observe the inhibitory effect of sachalin rhodiola rhizome extract (SRR) on regulatory T cells (Tregs) in xenograft tumors of Lewis lung cancer bearing mice and primarily discuss its mechanism of suppressing tumor growth. Methods: Lewis lung cancer-bearing mice were established and randomly divided into 3 groups: SRR group, paclitaxel (PTX) positive control group and PBS group. The changes of tumor volume were recorded in different groups, the tumor inhibition rates were calculated and the survival time of Lewis-bearing mice was observed. The proportion of CD4+CD25+Foxp3+Tregs in the xenograft tumor tissues was detected by flow cytometry. The mRNA expression levels of Foxp3 and TGF-β in the tumor tissues were detected by real-time PCR. Results: On day 20 after the establishment of the Lewis-bearing mouse model, the tumor volume of mice in the SRR group was significantly smaller than that in the PBS group (\[719.6±2.4\] vs \[1 030.5±3.1\] mm3, P<0.05), and showed no significant difference with the PTX positive control group (P>0.05). Compared with the PBS group, the survival time of mice in the SRR group was significantly prolonged (\[36.0±1.0\] vs \[22.0±2.0\] d, P<0.05), and showed no significant difference with the PTX group (P>005). The proportion of CD4+CD25+Foxp3+Tregs in CD4+T cells of the tumor tissues in the SRR group was significantly lower than that of the PBS group (\[8.5±0.3\]% vs \[11.2±0.2\]%, P<0.01), and no significant difference was observed between the SRR group and the PTX group (P>0.05). The mRNA expressions of Foxp3 (\[1.2±0.2\] vs \[21±0.2\], P<0.05) and TGF-β (\[1.2±0.2\] vs \[2.1±0.2\], P<0.05) in SRR group were significantly lower than that in the PBS group, and no significant difference was observed between the SRR group and the PTX group (P>005). Conclusion: SRR may enhance the antitumor immune response by down-regulating the proportion of CD4+CD25+Tregs and the mRNA expressions of Foxp3 and TGF-β in the tumor tissues.
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Project Supported:
Project supported by the Natural Science Foundation of Tibet Autonomous Region (No. 2011)
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