Mobile website
Archive > Volume 20 Issue 5 > 2013,20(5):522-528. DOI:10.3872/j.issn.1007-385X.2013.05.003 Prev Next
Prompt Report
Soluble Tie2 inhibits proliferation, migration and invasion of colonic cancer HCT116 cells through suppression of vascular mimicry formation
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To investigate the effect of soluble Tie 2 (sTie2) on the vascular mimicry (VM) formation, proliferation, migration and invasion of colonic cancer HCT116 cells. Methods: The recombinant plasmid pBLAST49-hsTie2 or control plasmid pBLAST49 was transfected into HCT116 cells by liposome to form hsTie2-HCT116 or Ctrl-HCT116 cells. 3D model culture, SRB, scratch and Transwell assay were conducted respectively to detect the VM formation, proliferation, migration and invasion of HCT116 cells. Western blotting was used to detect the expression of VE-cadherin protein. Results: The recombinant plasmid pBLAST49-hsTie2 was transfected into colorectal cancer HCT116 cells successfully. Compared with that in the Ctrl-HCT116 cells, the formation of VM (\[0.75±0.45\] vs \[7.50±052\], P<0.01) and the expression of VE-cadherin protein (\[1.23±0.08\] vs \[1.73±0.02\], P<0.01) in hsTie2-HCT116 cells was significantly decreased, and the survival rate was also significantly decreased (\[32.57±4.57\]% vs \[88.24±2194\]%, P<0.01). The migration (\[0.37±0.07\] vs \[0.80±0.03\] mm, P<0.01) and invasion capacity (\[5725±3.17\] vs \[127.25±6.25\], P<0.01) of HCT116 cells were inhibited significantly. Conclusion: sTie2 inhibits the proliferation, migration and invasion of colorectal cancer cells through suppression of the VM formation, which provides a good basis for the development of new drugs for the treatment of colorectal cancer by targeting both angiogenesis and VM formation.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China (No.30960455), and the Science and Technology Bureau Foundation of Yunnan Province (No.2008ZC142M)
Clc Number:
