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Archive > Volume 20 Issue 5 > 2013,20(5):547-551. DOI:10.3872/j.issn.1007-385X.2013.05.007 Prev Next
Basic Research
Effect of IL-2, IFN-α and IFN-γ on expression of B7-H4 in clear cell renal cell carcinoma 786-0 cells
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Abstract:
Objective : To explore the influence of IL-2, IFN-α and IFN-γ on the expression of B7-H4 in clear cell renal cell carcinoma 786-0 cells． Methods: Clear cell renal cell carcinoma 786-0 cells were stimulated by IL-2, IFN-α and IFN-γ for 24 h. The expression of B7-H4 mRNA was detected by RT-PCR. The expression of B7-H4 protein was detected by ELISA assay, cytoimmunochemistry assay and flow cytometry. Results: RT-PCR result showed that the expression of B7-H4 mRNA in IL-2 (0.75±0.06), IFN-α (0.68±0.05) and IFN-γ (0.95±0.08) group cells was significantly higher than that in the untreated group (0.30±0.03) (P<0.05). Immunocytochemistry showed that the expression of B7-H4 protein was detected both in the cell membrane and cytoplasm, and the expression of B7-H4 protein was up-regulated after stimulated by IL-2, IFN-α and IFN-γ. ELISA result showed that the expression of soluble B7-H4 protein in the supernatants of IL-2 (44.89±0.97) ng/ml, IFN-α (46.74±2.25) ng/ml and IFN-γ group cells (47.31±1.12) ng/ml was significantly higher than that in the untreated group (34.42±1.69) ng/ml (P<0.05). Flow cytometry assay result showed that the positive expression rate of B7-H4 in IL-2 (44.89±0.94)%, IFN-α (46.41±0.55)% and IFN-γ (54.18±1.42)% group cells were significantly higher than that in the untreated group (30.45±0.96)% (P<005). Conclusion: IL-2, IFN-α and IFN-γ can up-regulate the expression of B7-H4, in which IFN-γ has the highest capacity.
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Project Supported:
Project supported by the Appropriate Technical Transformation of Zhejiang（No.2013ZHB001）, and the Outstanding Scientific Research and Talent Cultivation of Zhejiang Cancer Hospital（No.2012YC004）
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