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Archive > Volume 20 Issue 5 > 2013,20(5):569-574. DOI:10.3872/j.issn.1007-385X.2013.05.011 Prev Next
Basic Research
Effect of ABCE1 gene silencing on apoptosis, proliferation, migration and invasion of human esophageal carcinoma EC-109 cells
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Abstract:
Objective: To investigate the effect of silencing ATP-binding cassette protein E1 ( ABCE1) gene expression by electroporation on apoptosis, proliferation, migration and invasion of human esophageal squamous carcinoma EC-109 cells. Methods: The siRNA sequence (ABCE1-siRNA) targeting ABCE1 and the negative control sequence (NC-siRNA) were constructed and transfected into EC109 cells to obtain ABCE1-EC109 and NC-siRNA-EC109 cells, respectively. The expressions of ABCE1 mRNA and protein were detected by RT-PCR and Western blotting, respectively. Flow cytometry was used to detect the cell cycle and apoptosis of EC109 cells. The proliferation, migration and invasion of EC109 cells were evaluated by CCK-8 assay, wound closure assay and Transwell assay, respectively. Results: Compared with the NC-siRNA-EC109 cells, the expression levels of ABCE1 mRNA and protein were significantly decreased in the ABCE1-EC109 cells (\[0.47±0.04\] vs \[0.67±0.05\], \[0.63±0.09\] vs \[0.86±0.11\], both P<0.05). Compared with the NC-siRNA-EC109 cells, the proliferation of ABCE1-EC109 cells was significantly decreased (\[2.20±0.10\] vs \[2.91±0.13\]，P<0.05), the number of ABCE1-EC109 cells arrested at G0/G1 phase was increased (\[76.5±3.1\] vs \[56.1±2.7\], P<0.05), the cell apoptotic rate was increased (\[15.46±3.12\] vs \[0.54±0.24\], P<0.01), and the migration and invasion abilities were significantly decreased (migration: \[8.12±0.23\] vs \[1.91±0.11\], P<005; invasion: \[42.56±4.68\] vs \[68.78±6.98\]，P<0.01). Conclusion: ABCE1 gene silencing by electroporation promotes the apoptosis of esophageal squamous carcinoma EC109 cells, and inhibits their proliferation, migration and invasion in vitro.
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