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Archive > Volume 20 Issue 5 > 2013,20(5):580-585. DOI:10.3872/j.issn.1007-385X.2013.05.013 Prev Next
Basic Research
Norcantharidin enhances cytotoxicity of IL-15 activated PBMCs on leukemic KG1a cells
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Abstract:
Objective: To explore whether norcantharidin (NCTD) can enhance the cytotoxicity of IL-15 activated peripheral blood mononuclear cells (PBMCs) on human acute myeloblastic leukemic KG1a cells and its underlying mechanism. Methods: The effect of NCTD on the proliferation of KG1a cells was detected by typan blue assay and CCK-8 assay. The effect of NCTD on the cell cycle of KG1a cells was examined by flow cytometry. The cytotoxicity of IL-15 activated PBMCs (IL-15-PBMCs) against NCTD treated-KG1a cells was detected by LDH releasing assay. The expressions of NKG2D (natural killer group 2 member D) ligands on KG1a cells were detected by flow cytometry. Results: NCTD effectively inhibited the proliferation of leukemic KG1a cells, in a time- (r=0.398，P=0.000) and dose-dependent manner (r=0.861，P=0.000), and arrested KG1a cell cycle at G2/M phase. NCTD within a concentration of 4.00 μg/ml has no obvious cytotoxicity on the IL-15 activated PBMCs (IL-15-PBMCs) (P>0.05). Compared with the control group, the cytotoxic rate of IL-15-PBMCs on 0.125 μg/ml NCTD treated-KG1a cells was significantly increased (donor A: \[37.44±5.78\]% vs \[9.33±1.69\]%, \[38.33±3.07\]% vs \[16.75±1.20\]%, P<0.05). NCTD treatment showed no effect on expressions level of NKG2D ligands on KG1a cell surface (P>0.05). Conclusion: NCTD can enhance the cytotoxicity of IL-15-PBMCs on leukemic KG1a cells, which is possibly related to the inhibition of proliferation of KG1a cells and cell cycle arrest in G2/M phase.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 30973454), and the Program of Ministry of Education for New Century Excellent (No. NCET-09-0087)
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