Mobile website
Archive > Volume 20 Issue 5 > 2013,20(5):586-589. DOI:10.3872/j.issn.1007-385X.2013.05.014 Prev Next
Clinical Research
Relationship of K-Ras and P53 gene mutations with development and postoperative recurrence of hysteromyoma
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective : To explore the relationship of K-Ras and P53 gene mutation with development and postoperative recurrence of hysteromyoma. Methods: Fifty-six patients with hysteromyoma undergoing myomectomy were selected from First People Hospital of Xining during June 2008 to June 2010. The hysteromyoma tissues and normal uterus tissues were obtained from patients. K-Ras and P53 gene mutations were analyzed by polymerase chain reaction-single strand conformation polymorphism analysis (PCR-SSCP) and gene sequencing. The postoperative recurrence rate after uterine fibroid surgery in patients with K-Ras and P53 gene mutation was analyzed. Results: K-Ras gene mutation was focused on exon 1 and 2. P53 gene mutation was focused on exon 7 and 8. The mutation rate of K-Ras and P53 and the double mutation rate in the hysteromyoma tissues were significantly increased when compared with those in the normal uterus tissues (73.21% vs 7.14%, 8393% vs 10.71%, 32.14% vs 1.79%, P<0.05). Postoperative 3-year cumulative recurrence rates were 14.28% (6/42) and 8.51% (4/47) in the hysteromyoma patients with K-Ras or P53 mutation. Postoperative 3-year cumulative recurrence rate in the patients with double mutation was 66.67% (12/18), which was significantly higher than that in patients with single mutation (P<0.05). Conclusion: K-Ras and P53 gene mutations may be one of the main reasons leading to the occurrence and postoperative recurrence of hysteromyoma, which can be indicators for clinical diagnosis and prognosis.
Keywords:
Project Supported:
Project supported by the Scientific Research Foundation of Qinghai Province (No.963211Y0141)
Clc Number:
