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Albendazole-mediated inhibition on invasion and migration of colon carcinoma SW480 cells and the underlying mechanisms
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Abstract:
Objective : To investigate the inhibitory effect of albendazole on colon carcinoma cell invasion and migration as well as the mechanisms underlying the effect. Methods: Colon carcinoma SW480 cells were treated with albendazole at 0 mg/ml, 1.0 mg/ml and 2.0 mg/ml. Cell proliferation was assessed by a cell proliferation assay using a cell counting Kit-8 (CCK-8), cell migration and invasion by wound-healing assays and transwell chamber assays, respectively, and protein levels of E-cadherin, MMP-2 and MMP-9 by immunocytochemistry and Western blotting. Results: Compared with the control (0 mg/ml), 1.0 mg/ml and 2.0 mg/ml albendazole significantly inhibited SW480 cell proliferation (P<005), and significantly decreased the invasion and migration in SW480 cells at both concentrations tested (P<0.05). Albendazole treatments significantly reduced the protein level of MMP-2 and MMP-9 but increased the protein level of E-cadherin. Conclusion: Albendazole can inhibit the invasion and migration of SW480 cells, at least partially through up-regulating the expression of E-cadherin and down-regulating the expression of MMP-2 and MMP-9.
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