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Clinical significance of S-phase kinase-associated protein 2 measurement in cervical squamous cell carcinoma tissuse
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Abstract:
Objective : To investigate the clinical significance of S-phase kinase-associated protein 2 (Skp2) measurement in the diagnosis and prognosis of cervical squamous cell carcinoma (SCC). Methods: Paraffin-embedded blocks of cervical tissue specimens collected from 25 healthy women, 84 women with cervical intraepithelial neoplasia (CIN) and 163 women with SCC who were cared in the Second People’s Hospital Affiliated to Shanghai Jiaotong University and Yanbian Women’s and Children’s Hospital between 2004 and 2008 were obtained. Protein content of Skp2 and the presence of human papillomavirus (HPV) in these specimens were analyzed by immunohistochemical staining and PCR, respectively. The correlation between Skp2 content and prognostic scores was also analyzed. Results: While Skp2 protein was undetectable in the 25 healthy control subjects, it was detected in 84.0% (137/163) of SCC patients, 37.9% (10/29) of CIN-1 patients, 81.6% (31/38) of CIN-2 patients and 82.4% (14/17) of CIN-3 patients (P<0.01). Skp2 protein content was closely related with HPV infection and the International Federation of Gynecology and Obstetrics (FIGO) clinical stage. The lesion-free survival and overall survival rates were 55.5% and 59.1%, respectively, in Skp2-positive patients and were 96.2% and 88.5% (Log-rank 11.530 and 10.154, P＝001), respectively, in Skp2-negative patients. Skp2 content in the cervical epithelium was not correlated with patient age, Ki-67 expression and pathological grade of cervical cancer. Conclusion: Skp2 may be a biomarker of cervical squamous cell carcinoma cell proliferation and therefore Skp2 protein content measurement may have a clinical significance in the diagnosis and prognosis of cervical cancer.
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Project Supported:
Project supported by the Natural Science Foundation of China (No.61371067), and the Projects of Research & Innovation of Jilin Youth Leader and Team (No.20130521017JH)
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