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Archive > Volume 21 Issue 2 > 2014,21(2):125-129. DOI:10.3872/j.issn.1007-385X.2014.02.002 Prev Next
Prompt Report
Role for E2F1 in the regulation of transcription factor dimerization partner-3 expression and apoptosis in prostate cancer PC3 cells
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Abstract:
Objective : To evaluate the regulatory effects of E2F1 on transcription factor dimerization partner-3 ( TFDP3 ) expression and apoptosis in prostate cancer cells in vitro. Methods: A luciferase reporter construct driven by the human TFDP3 gene promoter, pGL3-TFDP3-promoter, was made through PCR and subcloning using the total DNA extracted from human prostate cancer PC3 cells. PC3 cells were transfected with pGL3-TFDP3-promoter and pCMV-E2F1-HA E2F1 expression vector, either alone or together. TFDP3 promoter activity and TFDP3 protein content in the transfectants were determined by dual luciferase assays and Western blotting analysis, respectively, 48 h after transfection, while apoptosis was analyzed by flow cytometry 24 h after transfection. Results: The luciferase activity was significantly higher in PC3 cells co-transfected with pGL3-TFDP3-promoter and pCMV-E2F1-HA than PC3 cells transfected with pGL3-TFDP3-promoter alone (1.14 vs 0.61, P<0.05). TFDP3 protein content in PC3 cells transfected with pCMV-E2F1-HA was 2.7 times higher than that in non-transfected cells ([0.24±0.03] vs [0.089±0.02], P<0.05). The proportion of apoptotic cells PC3 cells transfected with pCMV-E2F1-HA (7.1±0.003)% was significantly higher than that both in non-transfected PC3 cells ([2.66±0.001]% P<0.05) and in PC3 cells co-transfected with pcDNA3-TFDP3-promoter and pCMV-E2F1-HA ([4.92±0.002]% vs [7.1±0.003]%，P<0.05). Conclusion: E2F1 may enhance the TFDP3 promoter activity and upregulate TFDP3expression in prostate cancer cells. This finding suggests that E2F1 and TFDP3 may play a role in the survival/apoptosis in prostate cancer cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No.81272619）
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