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Archive > Volume 21 Issue 2 > 2014,21(2):142-146. DOI:10.3872/j.issn.1007-385X.2014.02.005 Prev Next
Basic Research
Analysis on lymphocyte subsets in tumor-draning lymph nodes of esophageal cancer patients
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Abstract:
Objective : To investigate changes in lymphocyte subsets in tumor-draining lymph nodes (TDLNs) of esophageal cancer patients and in association with tumor progression. Methods: A total of 70 TDLN specimens were collected from esophageal cancer patients who underwent esophagectomy in the Fourth Hospital of Hebei Medical University. They were categorized as metastatic and non-metastatic based on their metastatic statuses, and as early stage and advanced stage according the TNM staging. Lymphocytes were isolated sterilely from these TDLN specimens. Proportions of CD3+ T cell, CD3+CD4+ T cell, CD3+CD8+ T cell, CD3-CD19+ B cell, CD3-CD16+CD56+ NK cell and CD4+CD25+ Treg in the mixed lymphocyte preparations were analyzed by flow cytometry. The relationships between Treg and other lymphocyte subsets were examined by Pearson’s correlation test. The differences in lymphocyte subset proportions were analyzed by student t test or Mann-Whitney U test. Results: The proportion of Treg was correlated with proportions of T cells (P=0.002) and CD4+ T cells (P=0.003) but not with proportions of CD8+ T cells, B cells and NK cells ( P>0.05). Compared to the non-metastatic group, proportions of T cells (P<0.001), CD4+ T cells (P<0.001), CD8+ T cells (P=0.016) and NK cells (P=0.038) in the metastatic group were significantly decreased, while proportions of B cells (P<0.001) and Treg (P=0.018) were significantly increased and the CD4+/CD8+ T cell ratio was not significant different (P=0.687). Proportions of T cells (P<0.001), CD4+ T cells (P=0.008), CD8+ T cells (P=0.027) and NK cells (P=0.022) were significantly lower specimens but B cell (P<0.001) and Treg (P=0.043) proportions were significantly higher in the advanced stage specimens than in the early stage specimens, while difference in the CD4+/CD8+ T cell ratio was not statistically significant (P=0.770). Conclusion: The distribution of lymphocytes seems to be disordered in TDLNs of esophageal cancer patients, thereby allowing for possible lymph node metastasis and tumor progression.
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Project Supported:
Project supported by the Young Scientists Fundation of the National Natural Science Foundation of China （No. 81201607）
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