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Archive > Volume 21 Issue 2 > 2014,21(2):160-164. DOI:10.3872/j.issn.1007-385X.2014.02.008 Prev Next
Basic Research
Effects of siRNA CD31-mediated PECAM-1 gene silencing on proliferation and VEGF expression in vascular endothelial cells
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Abstract:
Objective : To investigate the effects of siRNA CD31-targeted silencing of the platelet endothelial cell adhesion molecule 1 ( PECAM-1 ) or CD31 gene on VEGF expression and proliferation in endothelial cells. Methods: Murine hemangioendothelioma cells (EOMAs) were used as a model. They were transfected with naked siRNA CD31, siRNA CD31-FAM, a stable negative control (SNC) siRNA and Opti-Med as a blank control, respectively, using lipofectamin (RNAi-mate). After transfection, cell proliferation was assessed by MTT assays. PECAM-1 and VEGF mRNA and protein levels were determined by RT-PCR and Western blotting respectively. Results: PECAM-1 mRNA and protein levels and proliferative activity were all significantly lower in EOMAs transfected with naked siRNA CD31 and siRNA CD31-FAM than in EOMAs transfected with the SNC and Opti-MEM (P<0.01). As compared with the SNC, naked siRNA CD31 and siRNA CD31-FAM rested significantly higher rates of proliferation inhibition (P<0.01). Conclusion: The chemically synthesized 2’-O-methyl-siRNA CD31 may effectively silence the target gene PECAM-1 and inhibit proliferation in EOMAs, at least partially through a VEGF signaling-dependent mechanism.
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Project Supported:
Project supported by the Science and Technology Project of Wenzhou City（No. Y20100182）
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