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Archive > Volume 21 Issue 2 > 2014,21(2):165-168. DOI:10.3872/j.issn.1007-385X.2014.02.009 Prev Next
Basic Research
Effect of histone deacetylase inhibitor MS-275 on proliferation and apoptosis in human cervical carcinoma SiHa cells
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Abstract:
Objective : To investigate the effect of histone deacetylase (HDAC) inhibitor MS-275 on proliferation and apoptosis in human cervical carcinoma cells as well as the mechanisms underlying the effect. Methods: The human cervical carcinoma cell line, SiHa, was used as a model. SiHa cells were treated with histone deacetylase inhibitor MS-275 at 5, 10, and 20 μmol/L, respectively, for 48 h. The cell viability was assessed by MTT assays and the rate of cell apoptosis was determined by flow cytometry. Levels of acetyl histone H4 and p21 and p53 gene transcripts were analyzed by Western blotting and RT-PCR respectively. Results: MS-275 treatment resulted in an decrease in cellular growth activity and increases in apoptosis acetyl level of histone H4 and p21 and p53 mRNA abundance in SiHa cells in a concentration-dependent manner. Conclusion: Histone deacetylase inhibition may effectively inhibit the cellular proliferation and induce apoptosis in cervical carcinoma cells. The mechanisms underlying these effects may involve increased acetylation of histone up-regulated expression of p21 and p53.
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