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Archive > Volume 21 Issue 2 > 2014,21(2):169-174. DOI:10.3872/j.issn.1007-385X.2014.02.010 Prev Next
Basic Research
Effects of Quercetin on the proliferation and apoptosis of human small cell lung cancer H446 cells
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Abstract:
Objective : To observe the effect of Quercetin on the apoptosis of small cell lung cancer cell lines H446, and investigate the potential mechanism. Methods: After the treatment of 100 μmol/L and 200 μmol/L quercetin 48 h, confocal microscope was introduced to observe the effect of quercetin on proliferation of H446 cell. MTT assay was used to detect the anti-proliferative effect of quercetin on H446 cells. Flow cytometry was used to detect the influence of quercetin on the cell cycle of H446 cells. The expressions of apoptosis-related proteins P53, Bcl-2 and Bax in H446 cells were determined by Western blotting. Results: After treated with quercetin, nuclear became shrinkage and was divided into a serial of apoptotic bodies as the density of H446 cell decreased. Quercetin inhibited proliferation of H446 cells in a significant dose-dependent (P<0.05) and time-dependent (P<0.05) manner. After treated with quercetin for 12, 24, 48 and 72 h , its IC50 value to H446 cells were (172.2±2.6) , (102.4±5.3), (68.6±2.7) and (48.8±1.9) μmol/L respectively. Quercetin promoted the apoptosis of H446 cells in a significant dose-dependent manner. The apoptosis rate of H446 cell in 40 μmol/L quercetin group was higher than that of the control group ([8.3±0.4]% vs [4.0±0.5]%, P<0.01). When the concentration was arrived at 200 μmol/L, the apoptosis rate achieved the highest. Quercetin caused cell cycle arrest of H446 at the G2/M phase. Compared with the control group, the expressions of P53 ([4.98±0.91] vs [0.68±0.26], P<0.01) and Bax ([4.26±0.23] vs [0.89±0.29], P<0.01) were significantly higher in 200 μmol/L quercetin group, meanwhile, the Bcl-2 expression decreased significantly ([0.36±0.06] vs [8.23±1.65], P<0.01). Conclusion: Quercetin can inhibit the proliferation of H446 cells and promote it apoptosis, and the potential mechanism is probably related with regulation of apoptosis-related proteins such as Bax, P53 and Bcl-2.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81101758)
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