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Archive > Volume 21 Issue 2 > 2014,21(2):187-191. DOI:10.3872/j.issn.1007-385X.2014.02.013 Prev Next
Technigue and Method
Development and application of a modified chick embryo chorioallantoic membrane xenograft model of tumorigenesis in screening tumor angiogenesis inhibitors
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Abstract:
Objective : To modify the classical chick embryo chorioallantoic membrane (CAM) xenograft model of tumuorigenesis and evaluate the effectiveness and feasibility of the modified model in the screening of anti-angiogenesis drugs. Methods: Fertilized chicken eggs randomized into two groups. Eggs in the classical model group were grafted with U87 human glioma cells and the anti-angiogenic activity of test drugs, thalidomide (50, 100, 200 μg/ml), G3B6 and DMSO (the control) was determined by following the well-established classical methods. In the modified model group, the CAM was given an additional adaptation period of 9-16 h and then grafted with U8 cells by inoculating the cells into a silicone ring and then placing the ring on the half of the grade 1 vessel of the CAM. The test drugs were added into the silicone ring where their anti-angiogenic activity was evaluated. For both groups, the tumor morphology and the microvessel density (MVD) in the tumor tissue were examined under a stereo microscope with photos taken. Changes in the tumor histology was assessed by H-E staining and the expression of VEGFR2, the marker of the angiogenesis, was assessed by in situ hybridization. Results: The success rate of the xenograft was increased significantly without any negative effect on angiogenesis in the CAM in the modified model as compared with the classical model (\[70±4.226\]% vs \[41.25±5154\]%; t=4.314, P=0.000 7). The tumor volume was also significantly increased in the modified model group as compared with thecalissical model group (\[60.20±6.012\] vs \[15.97±2.403\] mm 3; t=6.012, P<0.000 1). Both thalidomide and G3B6 effectively inhibited the angiogenesis and reduced the MVD and VEGFR2 expression in the tumor tissue. Conclusion: The modified xenograft CAM model of tumuorigenesis developed in this study seems superior over the classical CAM model in studying tumorigenesis and screening anti-angiogenesis drugs.
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Project Supported:
Project supported by the Medicine Science Research Foundation of Guangdong Province（No. A2013312）and the National Natural Science Foundation of China (No. 31271455，No. 31200861，No. 31100852，No. 81200308,No.31200896)
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