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Archive > Volume 21 Issue 3 > 2014,21(3):282-287. DOI:10.3872/j.issn.1007-385X.2014.03.008 Prev Next
Basic Research
Effect and mechanisms of p-hydroxylcinnamaldehyde from cochinchina momordica seed on differentiation of mouse melanoma B16 cells in vitro
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Abstract:
To investigate the effect and underlying mechanisms of p-hydroxylcinnamaldehyde (PHD) isolated from the cochinchina momordica seed on the differentiation, proliferation and metastasis of mouse melanoma B16 cells in vitro. Methods: B16 cells were treated with PHD. At the designated time points after treatment, proliferation inhibition was assessed by the Suphrhodamine B assay, colony formation by plate colony assay, morphological changes by Giemsa staining, melanin content and tyrosinase activity by colorimetric analysis, metastasis by wound healing assays, and protein levels of Tyr, Trp1, t-proteins, p-p38, p-JNK and p-ERK1/2 by Western blotting. Results: PHD inhibited B16 cell proliferation in a dose-dependent manner (P<0.05). The inhibition rate at 20 μmol/L (37.70±2.28%), 40 μmol/L (42.17±4.18%) was even higher as compared with forskelin treatment (22.00±1.13%, P<0.05). B16 cells treated with PHD at 40 μmol/L for 24, 48, 72 h showed a dendrite-like morphology, indicative of differentiation. PHD (10 to 40 μmol/L) significantly increased melanin amount at 24 h (0.097±0.02), 48 h (0.13±0.04), and 48 h (0.15±005) as compared with non-treatment control (0.085±0.003, P<0.05) and tryosinase activity at 12 h (1.11±031), 24 h (1.43±0.05), and 48 h (1.67±0.49) as compared with control (0.64±0.10, P<0.05). PHD treatment effectively attenuated metastasis (P<0.05) and remarkably reduced colony-forming capacity (P<0.05) in B16 cells. Moreover, PHD significantly increased the protein content of Tyr, Trp1 and enhanced phosphorylation of P38 and but not ERK. Conclusion: P-hydroxylcinnamaldehyde may inhibit melanoma growth and metastasis, possibly through activating P38 and JNK signaling pathways.
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Project supported by the Medical Science Research Project of Hebei Province (No. 20120120)
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