Mobile website
Archive > Volume 21 Issue 3 > 2014,21(3):288-292. DOI:10.3872/j.issn.1007-385X.2014.03.009 Prev Next
Basic Research
Study on RIZ1 gene promoter methylation status in human glioblastoma cell lines
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
To investigate the methylation status in the promoter region of the retinoblastoma protein-interacting zinc finger1 (RIZ1) gene in four human glioblastoma (GBM) cell lines as well as the influence of methyltransferase inhibitor 5-Aza-CdR on RIZ1 gene transcription and proliferation in GBM cells with a hypermethylated RIZ1 promoter. Methods: The methylation status in the promoter region of RIZ1 in four human GBM cell lines, U87, U251, A172 and T98, were analyzed by methylation-specific polymerase chain reaction. The cell line with RIZ1 promoter hypermethylation was treated with 5-Aza-Cdr. In the treated cells, RIZ1 mRNA was assessed by real-time PCR and viability by MTT assays. Results: Promoter methylation of the RIZ1 gene was detected in GBM cell lines U87 and U251. The abundance of RIZ1 mRNA was significantly increased in U87 after treatment with 5-Aza-CdR. Treatment of U87 cells with 5-Aza-CdR resulted in a time- and concentration-dependent proliferation inhibition. Conclusions: Promoter methylation may play an important role in the epigenetic silencing of RIZ1 gene expression in human GBM cells.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China （No. 30930094），the National Natural Science Young Foundation of China （No. 81001118），the Foundation of Shanghai Municipal Health Bureau（No. 2009120），and the Foundation for Youths of Changzheng Hospital（No. 2012CZQN05）
Clc Number:
